Storage 

Store at -20°C. When stored as directed, product is stable for at least 12 months.

Description 

UEIris RT Reagent Kit is a simpler system for synthesizing RNA from cDNA. It contains all the reagents required for the first-strand cDNA synthesis, and only needs to add RNA template and water for reverse transcription. The synthesized cDNA is recommended for qPCR reaction.

Notes 

1. The purity and integrity of the RNA template is an important factor affecting the reverse transcription. 

2. For the subsequent experiment of qPCR, the amount of reverse transcription product should not exceed 1/10 of the volume of the qPCR system. 

3. The mix contains Oligo (dT) 20VN and random primers. It is not only suitable for eukaryotic mRNAs containing Poly(A) structure, but also for prokaryotic RNA without Poly(A) structure, eukaryotic rRNA, and tRNAs. But it is not suitable for small RNA templates such as miRNA.