Storage 

Store at 4℃ and protect from light. When stored as directed, product is stable for at least 6 months. Do not freeze

Spectral Characteristics 

YF ®488-Annexin V: Abs/Em = 490 /515 nm RedNucleus II: Abs/Em = 635 /695 nm (with DNA)

Description 

YF ®488 and RedNucleus II Apoptosis Kit provides a convenient method to make a distinction between apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Fluorescent conjugates of Annexin V can be used to label apoptotic cells.Human anticoagulant Annexin V is a 35-36 kilodalton, Ca 2+ -dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it is available for binding to fluorescently labeled Annexin V, which can be detected by fluorescence microscopy or flow cytometry.

RedNucleus II provided in this kit is a far red fluorescence dye, belonging to anthraquinone compounds, which can not penetrate the intact cell membrane of living cells and early apoptotic cells, and is impermeable, but can rapidly stain nucleus/dsDNA in dead and permeable cells. RedNucleus II is an ideal substitute of propidium iodide (PI) and 7-AAD. It can be used in multicolor analysis with FITC, PE and purple fluorescent dyes because it is not excited by ultraviolet and overlapped with PE/PE homologues without compensation controls. When combined with YF®488-Annexin V, RedNucleus II was excluded from living cells and early apoptotic cells, while late apoptotic cells and dead cells were stained with YF®488-Annexin V and RedNucleus II at the same time.

There is no spectral cross between YF ®488 and RedNucleus II, so there is no need to do compensation control for cells without spontaneous fluorescence, self-contained RFP or apoptosis cells induced by adriamycin.