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  • 2× SYBR Green qPCR Master Mix

    Product No.:S2014

    Product specification:20μL×100T/20μL×500T

    Catalogue price(RMB):250/600

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Product overview:

Product content




      A. 2×SYBR Green Master Mix   

1 mL

   5×1 mL   

B. 10 × ROX reference dye

   0.5 mL   

1 mL

The product contains two components. Component A contains SYBR Green dye, dNTP, PCR buffer (including Tris and MgCl2) and hot-start Taq DNA polymerase. Component B is 10× ROX reference dye, which may be required on certain ABI instruments (See protocol below).

Storage Conditions

Upon arrival, the SYBR Green qPCR Master Mix should be stored at -25°C to -15°C and protected from light. After each experiment, the leftover mix (completely thawed and thoroughly homogenized) can be stored at 4°C if it is to be used within the next 3 months. Avoid repeated freeze-thaw cycles to retain maximum performance. The SYBR Green qPCR Master Mix is stable for 2 years from the date of shipping when stored and handled properly.

Product Description

The 2×SYBR Green qPCR Master Mix is a ready-to-use cocktail containing all components (except primers and template) for the amplification and detection of DNA in qPCR. The regent is supplied as a 2× master mix with Taq DNA polymerase, dNTPs, MgCl2, fluorescent dye (detection), and proprietary buffer components. Furthermore, US Everbrigtht’s proprietary chemical modification of the DNA polymerase included in this Master Mix allows for Hot Start PCR, conferring a significant reduction in non-specific PCR amplification that is otherwise of a common occurrence with regular Taq polymerases.


1. ROX reference dyeFor certain instruments, ROX is necessary for obtaining accurate Ct value. The concentration of ROX can be referred to the following table 1. ROX will cause some background interference to the melting curve analysis. Therefore, in order to avoid ROX peak interference, do not select the ROX fluorescence value option in the “Passive Reference Dye” of the application software, and then collect and analyze the data.

2. Annealing temperature: The annealing temperature should be set at your primer’s Tm value, and it is usually 50-60 ℃ for optimal result. However, primer’s Tm value (and thus extension temperature) should be designed as closer as possible to 60 ℃ (but still within 50-60℃ range) to reduce the gap between annealing and denaturation temperatures. This requires less time for temperature increase and thus higher amplification efficiency.

3. For Real Time RT-PCR reactions, the reagents used for the reverse transcription reaction are recommended: UEIris II RT-PCR System for First-Strand cDNA Synthesis (no DNase I) (Catalog No. R2027), UEIris II RT-PCR System for First-Strand cDNA Synthesis (with DNase I) (Catalog No. R2028).



Citations and references:

 1.Genome-wide identifi cation and transcriptome-based expression analysis of sox gene family in the Japanese fl ounder Paralichthys olivaceus
YU Haiyang, DU Xinxin, LI Xiaojing, QU Jiangbo,ZHU He, ZHANG Quanqi, WANG Xubo
Journal of Oceanology and Limnology (2018)  

2. Whole-genome resequencing from bulked-segregant analysis reveals geneset based association analyses for the Vibrio anguillarum resistance of turbot(Scophthalmus maximus)

Zhang K, Han M, Liu Y, Lin X, Liu X, Zhu H, He Y, Zhang Q, Liu J.

Fish and Shellfish Immunology(2019)

3.Edwardsiella tarda-induced miR-7a functions as a suppressor in PI3K/AKT/GSK3β signaling pathway by targeting insulin receptor substrate-2 (IRS2a and IRS2b) in Paralichthys olivaceus


Fish and Shellfish Immunology(2019)

4.Duck RIG-I restricts duck enteritis virus infection

Hong Huo,Yue Wang,Dongfang Wang,Yiping Wang,Xiaohan Chen,Lili Zhao,Hongyan Chen.

Veterinary Microbiology(2019)

5.Comparative studies on duplicated tdrd7 paralogs in teleosts: Molecular evolution caused neo-functionalization

Bo Wang,Xinxin Du,Huizhen Wang,Chaofan Jin,Chen Gao,Jinxiang Liu,Quanqi Zhang

Comparative Biochemistry and Physiology Part D, genomics & proteomics(2019)

6.The Evolution and Possible Role of Two Sox8 Genes during Sex Differentiation in Japanese Flounder (Paralichthys olivaceus)

Haiyang Yu ,Yujue Wang ,Xiaojing Li,Feifei Ni,Minmin Sun,Quanqi Zhang,Haiyang Yu,Xubo Wang

Molecular Reproduction and Development(2019)

7.Inhibition of arsenite methylation induces synergistic genotoxicity of arsenite and benzo(a)pyrenediol epoxide in SCC-7 cells

Youxian Li,Man He,Beibei Chen,Bin Hu

Metallomics,11(1) · October 2018

8.Genome-wide identifi cation and transcriptome-based expression analysis of sox gene family in the Japanese fl ounder Paralichthys olivaceus

YU Haiyang;DU Xinxin;LI Xiaojing;QU Jiangbo;ZHU He;ZHANG Quanqi;WANG Xubo

Journal of Oceanology and Limnology(2018-05)

9.Evolutionary significance and regulated expression of Tdrd family genes in gynogenetic Japanese flounder (Paralichthys olivaceus)


Comparative Biochemistry and Physiology Part D, genomics & proteomics

10.Expansion and Evolutionary Patterns of Glycosyltransferase Family 8 in Gramineae Crop Genomes and Their Expression under Salt and Cold Stresses in Oryza sativa ssp. Japonica

Weilong Kong, Ziyun Gong, Hua Zhong, Yue Zhang, Gangqing Zhao, Mayank Gautam, Xiaoxiao Deng, Chang Liu, Chenhao Zhang and Yangsheng Li

Biomolecules 2019, 9(5), 188

11.Sugar Transporter Proteins (STPs) in Gramineae Crops: Comparative Analysis, Phylogeny, Evolution, and Expression Profiling

Weilong Kong, Baoguang An, Yue Zhang, Jing Yang, Shuangmiao Li, Tong Sun and Yangsheng Li 

Cells 20198(6), 560

12.Rapid evolution of piRNA pathway and its transposon targets in Japanese flounder(Paralichthys olivaceus)


Comparative Biochemistry and Physiology Part D: Genomics and Proteomics(Volume 31, September 2019)

13.LGP2 plays a critical role in MDA5-mediated antiviral activity against duck enteritis virus


Molecular Immunology Volume 116, December 2019, Pages 160-166

14.Glypican-3 Enhances Reprogramming of Glucose Metabolism in Liver Cancer Cells

Gebing Yao, Jikai Yin, Qing Wang, Rui Dong,  Jianguo Lu

BioMed Research International Volume 2019, Article ID 2560650, 11 pages


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