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ROS-DHE
Product overview:
Parameters
Ex/Em: 355/420 nm
Molecular Formula: C21H21N3
Molecular Weight: 315
Storage
Store at -20°C and protect from light. When stored as directed, product is stable for at least 6 months.
Description
The superoxide indicator dihydroethdium, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. According to the production of red fluorescence in living cells, the amount and change of ROS content in cells can be detected. Dihydroethidium is mainly oxidized by superoxide anion ROS in the cell. It can be directly observed by flow cytometry or fluorescence microscope. It is a fast and simple classic method for detecting ROS in living tissue or cultured cells.
Notes
1. Dihydroethidium is easily oxidized in the light and air. Please keep it away from light.
2. The reagent can be used for the detection of cultured live cells in vitro, cultured or perfused tissues and frozen sections of tissues.
3. For different cells and tissues, the appropriate incubation time and concentration should be selected to observe change of ROS.
Related products:
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D1002-50mg | H2DCFDA / DCFH-DA | View details |
D1004-5mg | Dihydroethidium (Hydroethidine) | View details |