Parameters

Ex/Em: 355/420 nm  

Molecular Formula: C₂₁H₂₁N₃

Molecular Weight: 315 

Storage

Store at -20°C and protect from light. When stored as directed, product is stable for at least 6 months.

Description

The superoxide indicator dihydroethdium, exhibits blue-fluorescence in the cytosol until oxidized, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. According to the production of red fluorescence in living cells, the amount and change of ROS content in cells can be detected. Dihydroethidium is mainly oxidized by superoxide anion ROS in the cell. It can be directly observed by flow cytometry or fluorescence microscope. It is a fast and simple classic method for detecting ROS in living tissue or cultured cells.

Notes

1. Dihydroethidium is easily oxidized in the light and air. Please keep it away from light.

2. The reagent can be used for the detection of cultured live cells in vitro, cultured or perfused tissues and frozen sections of tissues.

3. For different cells and tissues, the appropriate incubation time and concentration should be selected to observe change of ROS.