Parameters

Appearance: Orange-red solid soluble in DMSO or DMF 
Ex/Em (MeOH): 482/497 nm
Molecular Formula: C₂₁H₂₁IN₂O₂
Molecular Weight: 460.31

Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

DiOC2(3) has been used for measuring membrane potentials in bacteria. DiOC2(3) penetrates the cytosol of eukaryotic cells. At concentrations below 100 nM, the dye accumulates primarily in mitochondria with active membrane potentials, and red emission increases due to dye stacking. DiOC2(3) stain intensity decreases when cells are treated with reagents that disrupt mitochondrial membrane potential.Cells stained with DiOC2(3) can be visualized by flow cytometry with blue excitation and green and red emissions. The reagent can be paired with other reagents, such as Annexin V–APC, for multiparametric study of vitality and apoptosis.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.