Parameters

Appearance: Red solid soluble in water
Ex/Em (in MeOH): 510/625nm
Ex/Em (in membranes): ~480/600 nm
Molecular Formula: C₃₀H₄₉Br₂N₃
Molecular Weight: 612

Storage

Store at 4℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

Nerve terminal probes are a series of cationic styryl fluorescent dyes used to track synaptic activity at neuromuscular junctions or synapses. These dyes usually have a lipophilic tail (two carbon chains) and a highly hydrophilic head with cations. NerveGreen series probes are dyes with a single double bond.

NerveGreenTM C4 is a fixable, activity-dependent fluorescent nerve terminal probe and is a useful tool for synapse studies where subsequent fluorescent immunochemistry is desired. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained. The nonspecific staining of cell-surface membranes can simply be washed off prior to viewing.