Parameters

Appearance: Orange-red solid soluble in DMSO, DMF or MeOH 

Ex/Em (MeOH): 505/534 nm
Molecular Formula: C₂₁H₁₇ClN₂O₃
Molecular Weight:381

Storage

Store at -20 ℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells. In normal cells, it can rely on the mitochondrial transmembrane potential to enter the mitochondrial matrix, and the fluorescence intensity decreases or disappears. When apoptosis occurs, the integrity of the mitochondrial membrane is destroyed and the membrane permeability transport pores are opened. The membrane potential is reduced, and Rhodamine 123 releases mitochondria again, which emits strong yellow-green fluorescence. Rhodamine can detect the change of mitochondrial or purified mitochondrial membrane potential by fluorescence microscope, fluorescence photometer or flow cytometer.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.