Parameters

Appearance: Red solid soluble in DMSO or DMF

Ex/Em: 490/523 nm (in MeOH)

Storage

Store at -20 ℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

MitoScene™ Green II is a green fluorescent mitochondrial dye that stains mitochondria at the nanomolar level. The dye is non-fluorescent until it partitions into the mitochondrial membrane. The staining relies on mitochondrial mass, not on mitochondria membrane potential. Thus, the dye can be used to stain mitochondria in both live cells and fixed cells. When stained fixed cells, the signal-to-noise ratios is unideal. The fluorescent signal will be weakened or lost after fixed and permeabilized. 

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.