Parameters

Appearance: Red solid soluble in DMSO or MeOH 
Ex/Em (with DNA): 528/617 nm

Abs (no DNA): 493 nm

Molecular Formula: C₄₆H₅₀C_l4N₈
Molecular Weight: 857

Storage

Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description

EthD-I is a high-affinity fluorescent nucleic acid dye that can be used on mammals, bacteria, yeast and fungi. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. EthD-I is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.