Contents:

Storage

Component A, B: Store at 4℃ and protect from light, avoid freeze-thawing; Component C: Store at -20℃. When stored as directed, product is stable for at least 12 months.

Parameters

Ex/ Em: 510/527 nm (Monomer, green fluorescence)

585/590 nm (Aggregation, red fluorescence)

Description

JC-1 is a membrane permeable dye widely used for  determining mitochondrial membrance potential with flow cytometry or fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm to 590 nm when excited at 488 nm. Both colors can be detected using FITC and PE filter, respectively. JC-1 is qualitative in regards to the shift from green to red fluorescence emission, and and can be quantitatived as measured by fluorescence intensity in both filter sets. JC-1 can be used to indicate the initiation of apoptosis.