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  • WonderOrange™ Protein Quantitation Kit

    Product No.:W6006

    Product specification:100T/1000T

    Catalogue price(RMB):135/1085

    Can replace similar products:BCA, Bradford and Lowry, NanoOrange Protein Quantitation Kit

    Bulk Request


Product overview:

Contents:

Component 100 T 1000 T
A. WonderOrange™ buffer, 10× (contains 2 mM sodium azide) 2.5 mL 25 mL
B. WonderOrange™ dye,200×in DMSO 125 μL 1.25 mL
C. Bovine serum albumin (BSA) standard, 2mg/mL (contains 2 mM sodium azide) 40 μL 0.4 mL
T(Times) is based on 96 well plate calculation.

Storage

Component A: Store at room temperature; Component B: Store at room temperature and protect from light. Component C: Store at 4℃. When stored as directed, product is stable for at least 6 months.

 

Parameters

Ex/ Em: 480/598 nm (combined with BSA)


Description

WonderOrange™ Protein Quantitation Kit is a highly sensitive kit based on fluorescence technology for quantitative purification of protein, and its detection concentration range is 0.1-10 μg/mL. Compared with traditional methods such as BCA, Bradford or Lowry protein quantitative analysis, sensitivity of WonderOrange™ Protein Quantitation Kit is much better. In addition, compared with the NanoOrange protein quantitative analysis technology, it has better linearity and reproducibility . WonderOrange™ Protein Quantitation Kit can show the smallest variability between different proteins, and the fluorescence signal is stable for up to 16 hours; it applies to purified proteins and antibodies.

It is important to note that WonderOrange™ has varying degrees of tolerance to salts, buffers, detergents, or other chemicals .


Instructions:


    UE-W6006

Citations and references:


1.Increased mtDNA copy number promotes cancer progression by enhancing mitochondrial oxidative phosphorylation in microsatellite-stable colorectal cancer

Xiacheng Sun1, Lei Zhan2, Yibing Chen3, Gang Wang4, Linjie He, Qian Wang, Feng Zhou, Fang Yang, Jin Wu, Yousheng Wu,Jinliang Xing, Xianli He, Qichao Huang

Signal Transduct Target Ther (2018) DOI: 10.1038/s41392-018-0011-z

2.Mitochondrial fission promotes cell migration by Ca2+/CaMKII/ERK/FAK pathway in hepatocellular carcinoma

Xiacheng Sun,Haiyan Cao, Lei Zhan, Chun Yin, Gang Wang, Ping Liang, Jibin Li, Zhe Wang, Bingrong Liu, Qichao Huang, Jinliang Xing

liver international (2018) 1263-1272

3.DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response

Haichang Yin, Lili Zhao, Xinjie Jiang, Siqi Li, Hong Huo, Hongyan Chen

PLOS ONE (2017) DOI: 10.1371/journal.pone.0189704

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