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WonderOrange™ Protein Quantitation Kit
Product overview:
Contents:
T(Times) is based on 96 well plate calculation.
Component
100 T
1000 T
A. WonderOrange™ buffer, 10× (contains 2 mM sodium azide)
2.5 mL
25 mL
B. WonderOrange™ dye,200×in DMSO
125 μL
1.25 mL
C. Bovine serum albumin (BSA) standard, 2mg/mL (contains 2 mM sodium azide)
40 μL
0.4 mL
Storage
Component A: Store at room temperature; Component B: Store at room temperature and protect from light. Component C: Store at 4℃. When stored as directed, product is stable for at least 6 months.
Parameters
Ex/ Em: 480/598 nm (combined with BSA)
Description
WonderOrange™ Protein Quantitation Kit is a highly sensitive kit based on fluorescence technology for quantitative purification of protein, and its detection concentration range is 0.1-10 μg/mL. Compared with traditional methods such as BCA, Bradford or Lowry protein quantitative analysis, sensitivity of WonderOrange™ Protein Quantitation Kit is much better. In addition, compared with the NanoOrange protein quantitative analysis technology, it has better linearity and reproducibility . WonderOrange™ Protein Quantitation Kit can show the smallest variability between different proteins, and the fluorescence signal is stable for up to 16 hours; it applies to purified proteins and antibodies.
It is important to note that WonderOrange™ has varying degrees of tolerance to salts, buffers, detergents, or other chemicals .
Citations and references:
Xiacheng Sun1, Lei Zhan2, Yibing Chen3, Gang Wang4, Linjie He, Qian Wang, Feng Zhou, Fang Yang, Jin Wu, Yousheng Wu,Jinliang Xing, Xianli He, Qichao Huang
Signal Transduct Target Ther (2018) DOI: 10.1038/s41392-018-0011-z
Xiacheng Sun,Haiyan Cao, Lei Zhan, Chun Yin, Gang Wang, Ping Liang, Jibin Li, Zhe Wang, Bingrong Liu, Qichao Huang, Jinliang Xing
liver international (2018) 1263-1272
3.DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response
Haichang Yin, Lili Zhao, Xinjie Jiang, Siqi Li, Hong Huo, Hongyan Chen
PLOS ONE (2017) DOI: 10.1371/journal.pone.0189704
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