Product content

Spectral Properties of Super EvaGreen Dye:

The absorption and fluorescence emission spectra of DNA- bound Super EvaGreen dye are very similar to those of SYBR Green I or FAM.

λabs/λem = 500/530 nm  (DNA bound)

λabs = 471 nm (without DNA)

Storage and Handling

AugeGreen^TM qPCR Master Mix is shipped on blue ice and should be stored immediately upon arrival at -20℃. When stored under the recommended condition and handled correctly, the kit should be stable for at least 12 months from the date of receipt. Before use, thaw the product at room temperature (warm up directly without ice) and gently vortex to mix. Uneven mixing of reagents can result in poor experimental results. After thawing, all experiments should be performed on ice. The product can be refrigerated for storage.

Product Description

AugeGreen^TM qPCR Master Mix is a ready-to-use hot-start mix for qPCR and DNA melt curve analysis of PCR amplicons. It is formulated for qPCR using a fast cycling protocol, but also can be used for qPCR with regular cycling protocols.

AugeGreen^TM dye is a unique DNA-binding dye with features ideal for both qPCR and melt curve analysis. AugeGreen^TM dye binds to dsDNA via a novel “release-on-demand” mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition.

AugeGreen^TM qPCR Master Mix contains our proprietary chemically-modified UE hot-start Taq DNA Polymerase. Unlike AmpliTaq Gold, which is also a chemically modified Taq but takes 10 minutes or longer to activate, this UE Taq DNA Polymerase is fully activated in 2 minutes with high activity recovery, making it particularly suitable for fast PCR. UE HS-Taq is completely inactive at room temperature and largely free of DNA contamination. This makes UE HS-Taq superior to any antibody-based hot-start Taq, which is typically not completely inactive at room temperature and is prone to DNA contamination due to the nature of antibody production.

A unique feature of AugeGreen^TM dye is its safety. DNA-binding dyes are inherently dangerous due to their potential to cause mutation. With this in mind, our scientists designed AugeGreen^TM dye such that it cannot cross cell membranes, thus preventing the dye from being in contact with genomic DNA in live cells. All other commercial PCR dyes enter into cells in a matter of minutes. SYBR Green I, for example, has been shown to be environmentally more toxic than ethidium bromide, a well-known mutagen.

An added benefit of AugeGreen^TM Master Mix is that you can analyze your PCR product by gel electrophoresis without the need to add another DNA-binding dye to either your loading buffer or gel. The SAugeGreen^TM dye in the Master Mix can act as a DNA prestain, permitting direct visualization of DNA bands following electrophoresis.

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