Contents

Component A contains Super EvaGreen dye, dNTP, PCR buffer (including Tris and MgCl₂) and hot-start Taq polymerase. Component B is 10× Rox reference, which may be required on certain ABI instruments (See protocol below).

Parameters

abs/Em = 500/530 nm (DNA bound)

abs = 471 nm (without DNA)

Storage

Store at -20°C and protect from light. When stored as directed, product is stable for at least 6 months.

Description

AugeGreen^TM Master Mix for HRM is a professional kit for real-time quantitative PCR and high-resolution melting curve analysis. AugeGreen^TM dye binds to dsDNA via a novel “release-on-demand” mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition. It can make the binding amount of double-stranded PCR products reach the saturation, so it is called saturated dye and will not produce rearrangement like SYBR Green. Another unique feature of AugeGreen^TM is its safety.

This product contains a high-fidelity hot-start Taq DNA polymerase, which is chemically modified and has no activity at room temperature to avoid amplification of non-specific products. This kit can be used for SNP analysis, unknown mutation gene scanning and methylation detection.

A unique feature of AugeGreen^TM dye is its safety. DNA-binding dyes are inherently dangerous due to their potential to cause mutation. UE’s scientists designed AugeGreen^TM dye such that it cannot cross cell membranes, thus preventing the dye from being in contact with genomic DNA in live cells. All other commercial PCR dyes, such as SYBR^® Green I, enter into cells in a matter of minutes.