Parameters

Appearance: Off-white solid soluble in DMSO
Ex/Em: 492/517 nm (PH=9) (after hydrolysis)
Molecular Formula: C₂₅H₁₆O₉
Molecular Weight: 460.396

Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

6-CFDA (full name: 6-Carboxyfluorescein diacetate) is a live cell fluorescent tracer probe with membrane permeability and thus can be loaded into cells via incubation. Once inside cells, the non-fluorescent CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein. These fluorescent products can only accumulate in cells with integral cell-membrane and be detected by fluorescence microscopy or flow cytometry.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.