Parameters

Appearance: Purple solid soluble in DMSO, DMF or EtOH 
Ex/Em: 553/570 nm

Molecular Formula: C₆₈H₁₀₅Cl₂N₃O
Molecular Weight: 1051.5

Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

Cell Tracker^TM CM-DiI is a derivative of DiI and has better solubility in water than DiI. Cell Tracker™ CM-DiI fluorescent dye has been designed to freely pass through cell membranes into cells, where it is transformed into cell membrane- impermeant reaction products. Cell Tracker™ CM-DiI dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. Cell Tracker™ CM-DiI dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties: it is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of Cell Tracker™ CM-DiI dye are well separated from GFP (green fluorescent protein) spectra allowing for multiplexing. 

Cell Tracker^TM CM-DiI incorporates a mildly thiol-reactive chloromethyl substituent that confers aldehyde fixability via conjugation to thiol-containing peptides and proteins. Unlike other cell membrane dyes, Cell Tracker^TM CM-DiI is retained in cells throughout fixation, permeabilization, and paraffin embedding procedures.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.