Parameters

Appearance: Dark blue solid soluble in DMSO, DMF or EtOH 
Ex/Em (MeOH): 644/663 nm

Molecular Formula: C₆₇H₁₀₃ClN₂O₃S
Molecular Weight: 1052.1

Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

DiD is cyanine fluorescent dye with hydrophobic hydrocarbon tails, which can be used to stain cell membranes and other fat-soluble biological structures. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state life times (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

DiD is similar to DiI, but with longer absorption and emission wavelengths which is more valuable in cell and tissue staining. Cells can be fixed with formaldehyde either before or after staining, but not recommended for permeabilization.

Notes

1. When DiD stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background.

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

3. For your safety and health, please wear lab coats and disposable gloves.

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