Parameters

Appearance: Orange yellow solid soluble in ethanol, hexane, DMSO or vegetable oil
Ex/Em (MeOH): 484/501 nm

Molecular Formula: C₁₆H₁₇Cl₂N₅
Molecular Weight: 1086

Storage

Store at 4℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate. Neuro-DiO and DiO have nearly identical absorption and emission spectra, but the former has better solubility in membranes and does not form nonfluorescent aggregates, which also tend to slow down the dye diffusion rate in membranes.

Cells can be fixed with formaldehyde either before or after Neuro-DiO staining, but not recommended for permeabilization.

Notes

1. When Neuro-DiO stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background.

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

3. For your safety and health, please wear lab coats and disposable gloves.