Parameters

Appearance: Red solid soluble in DMSO 
Ex/Em (MeOH): 491/613 nm

Molecular Formula: C₄₆H₇₉IN₂
Molecular Weight: 787

Storage

Store at 4 ℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description

DiA is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. DiA and DiI have been used together for two color membrane staining.

Cells can be fixed with formaldehyde (Do not use methanol or other fixatives) either before or after staining, but not recommended for permeabilization. The effect of fixed cells by DIA staining was better than that by DIO staining.

Notes

1. When DiA stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background.

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

3. For your safety and health, please wear lab coats and disposable gloves.