Parameters

Appearance: Dark red solid soluble in DMSO, DMF or EtOH 
Ex/Em (MeOH): 549/565 nm

Molecular Formula: C₅₉H₈₉ClN₂O₄
Molecular Weight: 925.82

Storage

Store at 4 ℃and protect from light. When stored as directed, product is stable for at least 12 months.

Description

Dilinoleyl DiI, also known as FAST DiI, has identical absorption and emission spectra as DiI. Dilinoleyl DiI reportedly migrates ~50% faster than DiI. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane. This property makes Dilinoleyl DiI particularly useful for tracing neurons in tissues.

Cells can be fixed with formaldehyde (Do not use methanol or other fixatives) either before or after staining, but not recommended for permeabilization.

Notes

1. When Dilinoleyl DiI stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background.

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

3. For your safety and health, please wear lab coats and disposable gloves.