Parameters

Ex/Em: 683/724 nm

Storage

Store at roomtemperature and protect from light. When stored as directed, product is stable for at least 12 months.

Description

CytoMBrite™ NIR680 Cytoplasmic Membrane Dye is a novel near-infrared carbocyanine dye for labeling the cytoplasmic membranes of living cells. The dye is used as tracer in cell-cell fusion, cellular adhesion, and migration applications several weeks due to their properties of low cytotoxicity and high resistance to intercellular transfer. The dyes have long 18-carbon hydrophobic tails and an additional water-soluble group. These unique chemical structure elements make the dyes easy to dissolve while providing highly stable cytoplasmic membrane staining, unlike traditional carbocyanine dyes like DiI, DiO, and DiR, which are often difficult to dissolve or prone to precipitation during cell staining. CytoMBrite™ NIR680 is compatible with both confocal microscopy and near-infrared imaging systems, also compatible with animal NIR imaging systems.

Cells can be fixed with formaldehyde either before or after CytoMBrite™ NIR680 staining, but not recommended for permeabilization. The dye is not suitable for bacteria or yeast.

Notes

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

2. For your safety and health, please wear lab coats and disposable gloves.