Parameters

NeuroDiO (C4048) Ex/Em: 484/501 nm

Storage

Store at -20°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description

DiI, DiO and DiD are a class of lipophilic carbon cyanine dyes. Carbocyanine dyes label cytoplasmic membrane and intracellular membrane structures efficiently and permanently. They have been used as tracers in cell–cell fusion, cellular adhesion, and migration applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. The combination of CytoMBrite Cytoplasmic Membrane Dyes and other cell membrane dyes, such as DiR, NIR680 provides an effective tool for multicolor imaging and flow cytometry.

Cells can be fixed with formaldehyde either before or after staining, but not recommended for permeabilization.

Notes

1. When CytoMBrite Cytoplasmic Membrane Dyes stain fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background.

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

3. For your safety and health, please wear lab coats and disposable gloves.